{"id":12707,"date":"2019-03-15T10:34:19","date_gmt":"2019-03-15T09:34:19","guid":{"rendered":"http:\/\/serveiseducatius.xtec.cat\/tarragones\/?p=12707"},"modified":"2019-04-27T08:45:33","modified_gmt":"2019-04-27T06:45:33","slug":"trics13_mfont","status":"publish","type":"post","link":"https:\/\/serveiseducatius.xtec.cat\/tarragones\/dinamitzacio\/trics\/trics13_mfont\/","title":{"rendered":"Efecte antiinflamatori del cacau"},"content":{"rendered":"

\"MariaT\u00edtol:\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/em><\/span><\/strong>Estudi de l’efecte antiinflamatori del cacau<\/strong>
\nAutor\/a:\u00a0\u00a0\u00a0\u00a0<\/span><\/em><\/strong> Maria Font Alberich<\/strong>
\nTutor\/a:\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/em><\/strong>Josepa de Maria Sierra
\nModalitat:\u00a0\u00a0<\/span><\/em><\/strong>Ci\u00e8ncies i tecnologia: Ci\u00e8ncies de la Salut
\n\u00c0rea:\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/em><\/strong>Bioqu\u00edmica
\nCentre:\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/em><\/strong>Ins Baix Camp
\nLocalitat:\u00a0 \u00a0 <\/span><\/em><\/strong>Reus<\/p>\n

Objectius:<\/strong><\/h6>\n
    \n
  1. Estudiar l\u2019efecte antiinflamatori del cacau i altres compostos bioactius a trav\u00e9s del marcador TNF-\u03b1 en c\u00e8l\u00b7lules on s\u2019ha indu\u00eft la inflamaci\u00f3 amb LPS, i incubades amb s\u00e8rums de voluntaris que havien ingerit crema de cacau o crema de cacau enriquida amb altres compostos bioactius.<\/li>\n
  2. Aprendre el m\u00e8tode cient\u00edfic quant a l\u2019elaboraci\u00f3 d\u2019un experiment des del disseny del mateix, passant per la seva realitzaci\u00f3 i acabant amb l\u2019avaluaci\u00f3 dels resultats que es duria a terme en la rutina d\u2019un laboratori d\u2019investigaci\u00f3.<\/li>\n
  3. Con\u00e8ixer, experimentar i manipular les t\u00e8cniques adients per dur a terme l\u2019experiment com el pipeteig, treball en condicions as\u00e8ptiques, descongelaci\u00f3 de c\u00e8l\u00b7lules, comptatge i viabilitat cel\u00b7lular, t\u00e8cnica com l\u2019Enzyme-linked immunosorbent assay (ELISA)<\/li>\n
  4. Determinar la citotoxicitat mitjan\u00e7ant la mesura de la secreci\u00f3 de la lactate dehydrogenase (LDH).<\/li>\n<\/ol>\n
    Hip\u00f2tesi:<\/strong><\/h6>\n

    La ingesta de cremes de cacau i cremes de cacau enriquides amb avellanes, fitosterols i fibra soluble tenen efecte antiinflamatori a trav\u00e9s de la reducci\u00f3 del marcador de inflamaci\u00f3 tumor necrosis factor \u03b1 (TNF-\u03b1), valorat en un model in vitro de mon\u00f2cits estimulats amb lipopolisac\u00e0rid (LPS).<\/p>\n

    El proc\u00e9s:<\/strong><\/h6>\n

    L’elaboraci\u00f3 del treball est\u00e0 estretament lligada a la realitzaci\u00f3 pr\u00e0ctica de l’investigaci\u00f3 en el laboratori, ja que \u00e9s un treball experimental en el que s’expliquen detalladament els m\u00e8todes i les t\u00e8cniques utilitzades al llarg de l’experiment. Posteriorment, s’observen els resultats i se n’elaboren els gr\u00e0fics corresponents. Se’n fa una discussi\u00f3 per tal de comparar els resultats entre s\u00ed i amb altres estudis. I finalment se n’extreuen les conclusions, respectant el m\u00e8tode cient\u00edfic.<\/p>\n

    Conclusions:<\/strong><\/h6>\n

    Aquest estudi mostra que els s\u00e8rums de persones que han ingerit cremes de cacau o cremes de cacau enriquides no tenen efecte antiinflamatori a trav\u00e9s de la reducci\u00f3 del marcador de inflamaci\u00f3 TNF-\u03b1 en un model in vitro de mon\u00f2cits estimulats amb LPS. Tot i que estudis previs mostren que la ingesta de la crema de cacau enriquida va disminuir la concentraci\u00f3 de la prote\u00efna C reactiva d\u2019alta sensibilitat, per\u00f2 no a trav\u00e9s de la disminuci\u00f3 del TNF-\u03b1 com s\u2019ha demostrat en aquest estudi. Podem concloure que aquesta crema de cacau enriquida tindria un efecte antiinflamatori per la via de la prote\u00efna C reactiva per\u00f2 no per la via del TNF-\u03b1.
    \nTamb\u00e9 he apr\u00e8s com es treballa en un laboratori professional i les bases del m\u00e8tode cient\u00edfic.<\/p>\n

    Bibliografia:<\/strong><\/h6>\n
      \n
    • Sol\u00e0 R, Valls RM, God\u00e0s G, Perez-Busquets G, Ribalta J, Girona J, Heras M,Cabr\u00e9 A, Castro A, Domenech G, Torres F, Masana L, Angl\u00e9s N, Reguant J, Ram\u00edrez B, Barriach JM.. Cocoa, hazelnuts, sterols and soluble fiber cream reduces lipids and inflammation biomarkers in hypertensive patients: a randomized controlled trial.<\/strong><\/span>.<\/li>\n
    • De Araujo QR, Gattward JN, Almoosawi S, Silva Md, Dantas PA, De Araujo J\u00fanior QR.. Cocoa and Human Health: From Head to Foot–A Review<\/strong><\/span>.<\/li>\n
    • Latif R. Health benefits of cocoa<\/strong><\/span>.<\/li>\n
    • Li F, Vijayasankaran N, Shen AY, Kiss R, Amanullah A. Cell culture processes for monoclonal antibody production<\/strong><\/span>.<\/li>\n
    • Kwok CS, Boekholdt SM, Lentjes MA, Loke YK, Luben RN, Yeong JK, Wareham NJ,Myint PK, Khaw KT.. Habitual chocolate consumption and risk of cardiovascular disease among healthy men and women.<\/strong><\/span>.<\/li>\n
    • Kerimi A, Williamson G.. The cardiovascular benefits of dark chocolate.<\/strong><\/span>.<\/li>\n
    • Vicente Pascual, Rosa M. Valls, Rosa Sol\u00e0.. Cacao y chocolate: \u00bfun placer cardiosaludable?<\/strong><\/span>.<\/li>\n
    • Crescenti A, Sol\u00e0 R, Valls RM, Caimari A, Del Bas JM, Anguera A,Angl\u00e9s N,Arola L.. Cocoa Consumption Alters the Global DNA Methylation of Peripheral Leukocytes in Humans with Cardiovascular Disease Risk Factors: A Randomized Controlled Trial.<\/strong><\/span>.<\/li>\n
    • Khan N, Khymenets O, Urp\u00ed-Sard\u00e0 M, Tulipani S, Garcia-Aloy M, Monagas M, Mora-Cubillos X, Llorach R, Andres-Lacueva C.. Cocoa polyphenols and inflammatory markers of cardiovascular disease.<\/strong><\/span>.<\/li>\n
    • Chanput W, Mes JJ, Wichers HJ. THP-1 cell line: an in vitro cell model for immune modulation approach.<\/strong><\/span>.<\/li>\n<\/ul>\n
      Presentaci\u00f3:<\/strong><\/h6>\n
      <\/h6>\n","protected":false},"excerpt":{"rendered":"

      Maria Font Alberich<\/p>\n","protected":false},"author":1,"featured_media":12710,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[391,70],"tags":[319,494],"class_list":["post-12707","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-ciencies","category-trics","tag-bioquimica","tag-trics_ins-baix-camp"],"post_mailing_queue_ids":[],"_links":{"self":[{"href":"https:\/\/serveiseducatius.xtec.cat\/tarragones\/wp-json\/wp\/v2\/posts\/12707","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/serveiseducatius.xtec.cat\/tarragones\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/serveiseducatius.xtec.cat\/tarragones\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/serveiseducatius.xtec.cat\/tarragones\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/serveiseducatius.xtec.cat\/tarragones\/wp-json\/wp\/v2\/comments?post=12707"}],"version-history":[{"count":3,"href":"https:\/\/serveiseducatius.xtec.cat\/tarragones\/wp-json\/wp\/v2\/posts\/12707\/revisions"}],"predecessor-version":[{"id":12711,"href":"https:\/\/serveiseducatius.xtec.cat\/tarragones\/wp-json\/wp\/v2\/posts\/12707\/revisions\/12711"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/serveiseducatius.xtec.cat\/tarragones\/wp-json\/wp\/v2\/media\/12710"}],"wp:attachment":[{"href":"https:\/\/serveiseducatius.xtec.cat\/tarragones\/wp-json\/wp\/v2\/media?parent=12707"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/serveiseducatius.xtec.cat\/tarragones\/wp-json\/wp\/v2\/categories?post=12707"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/serveiseducatius.xtec.cat\/tarragones\/wp-json\/wp\/v2\/tags?post=12707"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}